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Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).


Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.


Labeling with Cyanine3 maleimide is selective, and efficient.


We recommend using water-solubleSulfo-Cyanine3 maleimidefor the labeling of antibodies and other sensitive proteins.