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IPTG, CAS#:367-93-1
Catalog # | Pkg Size | Price | Quantity | Shopping Cart |
---|---|---|---|---|
R-S-9003 | g | ¥ 800 | 加入購物車 |
結構式
外觀 | White powder |
分子量 | 238.31 |
溶解度 | 有機溶劑或水 |
CAS號 | 367-93-1 |
質量控制 | 95% |
儲存條件 | -20℃ |
保存時間 | 1 Year |
其他信息 | 異丙基1-硫代-β-D-半乳糖吡喃糖苷 |
異丙基1-硫代-β-D-半乳糖吡喃糖苷(IPTG)是β–半乳糖苷酶的活性誘導物質,常用于藍白斑篩選及IPTG誘導的細菌內的蛋白表達等。當pUC系列的載體DNA(或其他帶有lacZ 基因載體DNA)以lacZ 缺失細胞為宿主進行轉化時、或用M13噬菌體的載體DNA進行轉染時,如果在平板培養基中加入X–Gal和IPTG,由于β–半乳糖苷酶的α–互補性,可以根據是否呈現白色菌落(或噬菌斑)而方便地挑選出基因重組體。此外,它還可以作為具有lac或tac等啟動子的表達載體的表達誘導物使用。
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